OST /lab

OST / LAB · Single-cell bioinformatics

An independent research program for deriving cell identity from data

This laboratory grew out of the research line opened by my 2024 MSc thesis, “Next-generation Cell Type Annotation: Integrating NLP and ML Techniques for Enhanced scRNA Classification.” Since completing the thesis, I have continued researching cell-type annotation here independently, without institutional affiliation or status as an official continuation of the thesis project.

Generate explainable preliminary annotations for PBMC cells by evaluating marker genes together with expression profiles.

This release is not a reproduction of the thesis NLP + autoencoder model. It is a transparent marker-enrichment baseline; learned thesis-derived models are on the research roadmap.

Cells / samples

16

Genes

42

Matched markers

42

Zero fraction

0%

Annotation results

Select a row to inspect cell-type scores and decision evidence.

SamplePredictionConfidenceCoverageStatus
PBMC_001CD4 T cell79.3%100%Confident
PBMC_002CD4 T cell73.5%100%Confident
PBMC_003Cytotoxic CD8 T78.6%100%Confident
PBMC_004Cytotoxic CD8 T75.3%100%Confident
PBMC_005B cell77.1%100%Confident
PBMC_006B cell78.6%100%Confident
PBMC_007NK cell79.6%100%Confident
PBMC_008NK cell79.5%100%Confident
PBMC_009CD14+ monocyte77.9%100%Confident
PBMC_010CD14+ monocyte84.4%100%Confident
PBMC_011FCGR3A+ monocyte77.8%100%Confident
PBMC_012FCGR3A+ monocyte75.5%100%Confident
PBMC_013Dendritic cell74.7%100%Confident
PBMC_014Dendritic cell72.2%100%Confident
PBMC_015Platelet74.8%100%Confident
PBMC_016Platelet75.9%100%Confident

Cell-type distribution

CD4 T cell2
Cytotoxic CD8 T2
B cell2
NK cell2
CD14+ monocyte2
FCGR3A+ monocyte2
Dendritic cell2
Platelet2

PBMC_001

CD4 T cell

Confident

Alternative cell types

CD4 T cell
79%
B cell
5%
CD14+ monocyte
4%
Cytotoxic CD8 T
4%

Decision evidence

Positive contributions support the prediction; negative contributions are evidence against that cell type.

IL7R

+1.35

CCR7

+1.17

LTB

+0.95

CD3D

+0.93

CD3E

+0.90

MALAT1

+0.31

NKG7

-0.03

GNLY

-0.03

OST Lab | Single-cell Bioinformatics Research